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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Int J Med Microbiol. 2014 Apr 28;304(0):637–644. doi: 10.1016/j.ijmm.2014.04.008

Fig. 1.

Fig. 1

Effects of psm-mec on virulence gene expression. (A) Expression of selected virulence-associated genes in wild-type, psm-mec deletion and psm-mec and fudoh start codon deletion mutants in strains MSA3407 and MSA890. wt, wild-type strain; Δpsm-mec, psm-mec gene deletion strain; psm-mec*, strain harboring a psm-mec start codon mutation abolishing translation; fudoh*, fudoh (hypothetical) start codon mutation abolishing translation of hypothetical Fudoh protein. (B) Corresponding growth curves. (C) Effect of agr and agr/psm-mec deletion on spa transcription in strain MSA3407. (A, C) Gene expression was determined using qRT-PCR in late exponential phase (8-h) cultures. Values are from measurements performed at least in triplicate (three biological replicates, measured three times each) and relative to expression of the housekeeping gene gyrB. **, p<0.01; ***, p<0.001; ****, p<0.0001.