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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Cytotherapy. 2014 May 16;16(8):1121–1131. doi: 10.1016/j.jcyt.2014.02.012

Figure 1. Generation of IL13 mutein-CAR T cells.

Figure 1

(A) Scheme of IL13 mutein-CARs. All four constructs consisted of an IgG1-CH2CH3 domain, a CD28 transmembrane (TM) domain, and costimulatory domains derived from CD28 and the CD3 ζ-chain. The binding domain contained different IL13 muteins with one (E13K or E13Y) or two amino acid substitution (E13K.K105R; E13Y.K105R). (C,B) CAR expression was confirmed using FACS analysis. Representative plots (B) and summary data (C) is shown (mean 80.2% – 85.6%, n=7-14 per CAR construct).