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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Cytotherapy. 2014 May 16;16(8):1121–1131. doi: 10.1016/j.jcyt.2014.02.012

Figure 2. IL13 mutein-CAR T cells release cytokines after stimulation with recombinant IL13Rα1 and IL13Rα2 proteins.

Figure 2

IL13 mutein-CAR or non-transduced (NT) T cells were stimulated with recombinant IL13Rα1, IL13Rα2, or IL4R proteins. After 24h IFNγ (A) or IL2 (B) was measured by ELISA (n=3). T cells expressing all four CAR constructs expressed significant levels of IFNγ (p<0.001) when stimulated with recombinant IL13Rα2 or IL13Rα1 proteins in comparison to IL4R stimulated T cells. Only stimulation with recombinant IL13Rα2 protein induced significant levels of IL2 (p<0.001).