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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Tissue Eng Regen Med. 2013 Apr 1;9(11):E13–E26. doi: 10.1002/term.1736

Figure 7. BIO-treated MSCs Retain Multipotent Potential in 3D.

Figure 7

Differentiation of BIO-treated encapsulated MSCs (A). BIO-treated MSCs underwent proliferation in 2D for 3 weeks before encapsulation and subsequent induction of osteogenesis (von Kossa, hydroxyapatite), chondrogenesis (toluidine blue, glycosaminoglycans), and adipogenesis (oil red o, lipid droplets) (Bar = 50 μM) (B). Subsequent gene analysis for markers of osteogenesis (Cbfa1) (Ci), chondrogenesis (Sox9) (Cii), and adipogeneis (PPARγ) (Ciii) normalized to both the housekeeping gene β-actin and undifferentiated 0 μM controls (*p<0.05, **p<0.01 vs. 0 μM) (mean +/− SD).