Table 1.
List of primers used to amplify the desired genes
| Primer name | 5′——————— < 3′ | Primer length | |
|---|---|---|---|
| IL-2 |
Forward |
CCTGAGCAGGGAGAATTACA |
20 |
| Reverse |
TCCAGAACATGCCGCAGA |
18 |
|
| Fas |
Forward |
GAGAATTGCTGAAGACATGACAATCC |
26 |
| Reverse |
ATGGCTGGAACTGAGGTAGTTTTCAC |
26 |
|
| Akt1 |
Forward |
CCTTTATTGGCTACAAGGAACGG |
23 |
| Reverse |
GAAGGTGCGCTCAATGACTG |
20 |
|
| CD28 |
Forward |
GTATTCCTACAACCTTCTCGCAA |
23 |
| Reverse |
GGGGCTGATAGGTAAAATTCCCA |
23 |
|
| Cdc42 |
Forward |
TTCTGGTTGTGTTTCAACTGCT |
22 |
| Reverse |
CCTCCCTTGGACTGCATCTG |
20 |
|
| Inf- γ |
Forward |
GCCCAATATCTCGGATGCTTC |
21 |
| Reverse |
GCCAAAATAGCTTCGGTAATCCT |
23 |
|
| TNF-α | Forward |
CCAACATGCTGATTGATGACACC |
23 |
| Reverse | GAGAATGCCAATTTTGATTGCCA | 23 | |