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. Author manuscript; available in PMC: 2014 Jul 9.
Published in final edited form as: Methods Mol Biol. 2013;1022:29–39. doi: 10.1007/978-1-62703-465-4_3

Fig.1. SDS-PAGE analysis of recombinant Gtf3 protein after purification by HisTrap™ Column and HiTrap™ Q column.

Fig.1

6XHis tagged recombinant Gtf3 was purified on HisTrap™ Column and eluted (Lanes 1 to 5) as described in the section 3.2. Cleavage and dialysis were performed overnight at 4 °C with SUMO protease. Following the dialysis, 6xHis-SUMO and 6xHis tagged SUMO protease were removed from the flow through by passing through a HisTrap™ Column. The flow through (Lane 7) was further purified using HiTrap™ Q column and eluted (Lane 8). Protein samples were analyzed on SDS-PAGE after each step (small aliquots were used). Lane 6, BenchMark™ Protein Ladder.