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. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Cell. 2014 Jun 19;157(7):1685–1697. doi: 10.1016/j.cell.2014.04.038

Figure 1. GR Ligand Binding Activity In Vitro Is Hsp90 Independent.

Figure 1

A) Equilibrium binding of 20nM F-dex to GRLBD measured by fluorescence polarization (±SD). Binding curve fit a KD of 154±14nM (±SEM).

B) Equilibrium binding with 5mM ATP/MgCl2. GRLBD alone (blue) and with 5µM Hsp90 (red) (±SD).