Skip to main content
. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Cell. 2014 Jun 19;157(7):1685–1697. doi: 10.1016/j.cell.2014.04.038

Figure 5. Cryo-EM Reconstruction of Hsp90, Hsp70, HOP, GRLBD Complex.

Figure 5

A) Cryo-EM reconstruction of Hsp90, Hsp70, HOP, GRLBD complex filtered to 38Å.

B) Cryo-EM reconstruction as in A with placement of Hsp70 NTD (3ATU) and SBD (1DKX) in cyan, and Hsp90 NTD rotated HOP model (Southworth and Agard, 2011) in magenta. Client binding site residues for E. coli Hsp90; E466, W467, N470 shown in red and M546, M550, L553, F554 in blue (Genest et al., 2013).

C) Top view shows density for the linker between Hsp70 domains (cyan).

D) Top view showing positioning of Hsp70 NTD between Hsp90 NTDs. See also Figure S5 and S6.