Figure 3.

TALEN generation of Calsyntenin-1 mutants. A, TALENs were designed against the second exon of Clstn-1. B, clstn-1^uw7and clstn-1^uw8 result in nucleotide deletions with a frame shift and predicted premature stop. clstn-1^uw9 has a 6 bp deletion and 9 bp insertion, which does not result in a frame shift. C, D, RB neurons labeled with HNK-1 antibody in wild-type (C) and clstn1^uw7/uw7 mutant (D) embryos from incross of clstn1^uw7/+ heterozygous fish. E, Quantification of axon branches crossing the horizontal myoseptum in wild-type (+/+), clstn1^uw7/+ heterozygous, and clstn1^uw7/uw7 homozygous embryos. **p = 0.004 (unpaired, two-tailed t test). Scale bar, 40 μm.