Figure 5.

Clstn-1 intracellular domain or CA-Rab5c expression reduces peripheral RB branching. A, Schematic showing structure of Clstn-1 and a DNA construct used to express Clstn-1 ICD in RB neurons. TM, Transmembrane domain; 2A, self-cleaving peptide. B, C, Individually labeled RB neurons expressing either GFP-CAAX (B) or Clstn-1 ICD (C) showing failure of peripheral axon formation in Clstn-1 ICD-expressing cell. Arrows indicate central axons; arrowheads indicate peripheral axons. D, RB neuron expressing constitutively active Rab5c (CA-Rab5c) shows reduced branching. E, Quantification of peripheral axon tips per neuron. *p = 0.02 (unpaired, two-tailed t test). Scale bar, 40 μm.