Figure 7.

Clstn-1 knockdown reduces Rab5 vesicle delivery to branch points. A, Endosomes accumulate at branch points (open yellow arrowheads) and in growth cones (arrows) of peripheral axons in control embryos. B, Clstn-1 knockdown embryos show reduced accumulations at branch points. C, Quantification of GFP accumulation intensity change at branch points (intensity at 30 min after activation divided by intensity at 3 min after activation). *p = 0.03 (unpaired, two-tailed t test). D, E, Photoactivation in branch point (yellow circle) of control neuron (D) or Clstn-1 KD neuron (E). Signal is largely dispersed from the branch points by 20 min after PA. Arrowheads indicate vesicles that moved from branch point into axon branches. Left branch in D is outlined for clarity. F, Quantification of change in vesicle accumulation at branch points (volume at 20 min divided by volume at 2 min) shows no difference between control and Clstn-1 KD (p = 0.885). Scale bars, 20 μm. Time is minutes after photoactivation.