Table 1.

Peripheral axon branching is reduced by Clstn-1 knockdown^a

	Axons crossing myoseptum RB population (somites 8–13)	No. of RB neurons (somites 8–13)	Branches crossing myoseptum (individual RBs)	No. of peripheral axon tips (individual RBs)
Controls	—	15.6 ± 0.5*	—	—
Wild-type	9.5 ± 1.2b	—	1.6 ± 0.4h	10.7 ± 1.4j
Standard control MO	11.0 ± 1.8c	—	—	—
ATG MO	5.4 ± 0.6d	14.4 ± 0.5f	ND	ND
Splice MO	6.5 ± 0.7e	16.1 ± 0.7g	0.3 ± 0.2i	3.9 ± 0.9k

^aAll comparisons made with two-tailed, unpaired t test. ND, Not determined.

^bn = 12 embryos.

^cn = 21 embryos.

^dn = 29 embryos; p = 0.0012 compared with wild-type; p = 0.03 compared with standard control MO.

^en = 17 embryos; p < 0.0001 compared with wild-type; p = 0.0002 compared with standard control MO.

^fn = 25 embryos; p = 0.1 compared with pooled controls.

^gn = 7 embryos; p = 0.5 compared with pooled controls.

^hn = 17 cells in 14 embryos.

ⁱn = 13 cells in 12 embryos; p = 0.005 compared with wild-type.

^jn = 15 cells in 13 embryos.

^kn = 8 cells in 7 embryos; p = 0.003 compared with wild-type.

*Pooled uninjected wild-type and standard control morpholino controls; n = 15 embryos.