Figure 3.

Knocking down sAC abrogates BDNF-dependent reversal of MAG-mediated inhibition. P5 CGNs were isolated and plated on PLL overnight. Fresh Sato containing scrambled siRNA (250 nm) or sAC siRNA (100 and 250 nm) was added. Nine hours later, BDNF was added and incubated for a total of 24 h. A, Total lysate was collected for the input (loading control) and sAC was immunoprecipitated using anti-sAC R37 followed by Western blotting (WB) using a biotinylated anti-sAC R21 antibody. B, In addition, the cells were transferred onto monolayers of either MAG-expressing CHO cells or control CHO cells for 22 h. Results represent the percentage of the average length of the longest neurite over the control from 150 to 300 neurons ± SEM; n = 3, *p < 0.05, **p < 0.01.