Figure 5.

Expression of exogenous sAC in primary rat postnatal neurons promotes neurite outgrowth in the presence of MAG or myelin in vitro. Isolated rat P5–P7 CGNs were infected with lentivirus containing sAC (LV-sACt) or LacZ-expressing lentivirus (LV-LacZ) as a control, then transferred onto monolayers of either MAG-expressing CHO cells or control CHO cells (A) or onto purified myelin or PLL control (B). Neurons were fixed and stained for β-III-tubulin. Results represent the percentage of the average length of the longest neurite over the control ± SEM; n = 3,*p < 0.05, ***p < 0.001.