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. 2014 Jul 9;34(28):9441–9454. doi: 10.1523/JNEUROSCI.5314-13.2014

Figure 3.

Figure 3.

Immunohistochemical analysis of passively immunized α-syn tg mice with antibodies against FL α-syn or CT-truncated α-syn. To determine the effects of passive immunization on FL-α-syn (A–C) or CT-α-syn (D–F) accumulation, immunohistochemical analysis using species-specific antibodies was conducted. A, Top, Images of the neocortex and hippocampus of non-tg mice immunized with control antibody 27-1 and α-syn tg mice immunized with antibodies 27-1, 9E4, 1H7, 5C1, or 5D12. Sections were immunolabeled with an antibody against FL-α-syn. Bottom, Higher-magnification images of the neocortex of the sections above. Scale bar, 30 μm. B, Analysis of the levels of α-syn immunoreactivity in the neocortex, measured as optical density in sections labeled with the FL-α-syn antibody. C, Stereological analysis of the numbers of neocortical α-syn-immunoreactive neurons stained with the FL-α-syn antibody. D, Top, Images of the neocortex, hippocampus, and striatum of non-tg mice immunized with control antibody 27-1 and α-syn tg mice immunized with antibodies 27-1, 9E4, 1H7, 5C1, or 5D12. Sections were immunolabeled with the antibody SYN105 against CT-α-syn. Bottom, Higher-magnification images of the striatum of the sections showed above. Arrows highlight α-syn-positive granular-like structures. Scale bar, 5 μm. E, Analysis of the levels of α-syn immunoreactivity in the neocortex, measured as the percentage of neuropil area stained in sections labeled with the CT-α-syn antibody. F, Analysis of the levels of α-syn immunoreactivity in the striatum, measured as the percentage of neuropil area stained in sections labeled with the CT-α-syn antibody. Error bars represent ± SEM. ***p < 0.001 when comparing non-tg 27-1-immunized mice to α-syn tg 27-1-immunized mice; #p < 0.05; ##p < 0.01; ###p < 0.001 comparing α-syn tg mice immunized with 27-1 with α-syn tg mice immunized with 9E4, 1H7, or 5C1.