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. 2014 Jul 9;34(28):9441–9454. doi: 10.1523/JNEUROSCI.5314-13.2014

Figure 7.

Figure 7.

Passive immunization with CT α-syn antibodies improved synaptic pathology in α-syn tg mice. The effect of passive immunization with CT α-syn antibodies on synaptic markers was evaluated in the neocortex, striatum, and hippocampus of non-tg mice immunized with 27-1 or α-syn tg mice immunized with 27-1, 9E4, 1H7, 5C1, or 5D12. A, Brain sections were stained with an antibody against synaptophysin (red) or an antibody against MAP2 (green), and representative fluorescence images are shown. Arrows highlight synaptic loss in the hippocampus of α-syn tg mice. Scale bar, 250 μm. B, Analysis in neocortex of the percentage of area of the neuropil stained by the synaptophysin antibody. C, Analysis in striatum of the percentage of area of the neuropil stained by the synaptophysin antibody. D, Analysis in neocortex of the percentage of area of the neuropil stained by the MAP2 antibody. E, Analysis of the percentage of neuropil stained by the MAP2 antibody in the CA3 area of the hippocampus. F, Immunoblot analysis of the synaptic markers PSD-95 and synaptophysin. Actin was used as a loading control. G, Densitometric analysis of the PSD-95-immunoreactive bands. H, Densitometric analysis of the synaptophysin-immunoreactive bands. Error bars represent ± SEM. *p < 0.05 and ***p < 0.001 comparing non-tg 27-1-immunized mice with α-syn tg 27-1-immunized mice; #p < 0.05, ##p < 0.01, ###p < 0.001 comparing α-syn tg mice immunized with 27-1 to α-syn tg mice immunized with 9E4, 1H7, or 5C1.