Table 3.

Characteristics of shiga toxin-producing Escherichia coli (STEC) isolated from cattle feces and environmental samples in Gyeonggi-do, Korea

^*Twenty-eight STEC strains were isolated from a total of 253 cattle farm samples from 15 enrolled cattle farms by three different methods. For isolation of STEC O157, a conventional culture method based on SMAC agar media was used; while immunomagnetic beads coated with anti-O157 were used for the immunomagnetic separation (IMS) method. ^†A polymerase chain reaction (PCR)-based culture method was used for isolation of non-O157 STEC strains. From a total of 253 samples, 17 STEC-O157 positive samples were excluded from the PCR assay. ^‡Two strains with different serotypic and genotypic characterizations were isolated from the same sample. ^§Shiga toxin types were determined by mPCR and the mLAMP assay, which were concordant. UT: untested.