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. 2014 Jul 1;11:121. doi: 10.1186/1743-422X-11-121

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Copyright © 2014 Shi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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VMir prediction of HAV antigenome-encoded pre-miRNAs. (A) The candidate pre-miRNA with high scores is circled in red. The VMir analyzer program was used to predict candidate pre-miRNAs in the HAV antigenome with a cutoff score of 150 and a cutoff window counts of 35. The y-axis represents VMir scores, while the x-axis indicates the nucleotide position. (B) Secondary structures of the predicted candidate HAV pre-miRNAs. Mature miRNA that was experimentally validated in this study is highlighted and indicated as “novel miR-N1-3p”.