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. 2014 Jul 1;11:121. doi: 10.1186/1743-422X-11-121

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Copyright © 2014 Shi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Knockdown of Dicer reduces the expression of HAV antigenome-encoded hav-miR-N1-3p. (A) Dicer mRNA was knocked down by RNAi and confirmed by qRT-PCR. (B) Dicer protein was knocked down by RNAi and confirmed by Western blot. Actin was used as a loading control. (C) Expression difference analysis of HAV antigenome-encoded hav-miR-N1-3p between Dicer-silencing and negative siRNA-transfected cells. HAV antigenome-encoded hav-miR-N1-3p expression changes were calculated relatively to U6 snRNA, and cellular miR-154 was used as a positive control. Significant difference in expression levels were detected by Student’s t-test (*P < 0.05 and **P < 0.01).