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. 2014 Jul 1;14:213. doi: 10.1186/1472-6882-14-213

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Copyright © 2014 Abdul Rahman et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of apoptosis using flow cytometry after caspase-3 antibody staining. 1321N1, SW1783 and LN18 cells were treated with combined treatments at IC50 concentrations for 24 h. (A) An example of active caspase-3 detection diagram from 1321N1 cells: viable cells are in the left quadrant, while the presence of active caspase-3 is shown in right quadrant (P2). (B) Combined compounds caused greater inhibition of growth of 1321N1, SW1783 and LN18 cells than either agent alone as evidence by the presence of active caspase-3. Each value represents mean ± SD of three independent experiments. *P < 0.05 compare to control. # P < 0.05 compare to GTT. $P < 0.05 compare to HC.