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. 2006 Nov 14;12(42):6812–6817. doi: 10.3748/wjg.v12.i42.6812

Figure 5.

Figure 5

Representative liver sections stained with TUNEL assay after 30 min of ischemia (pP0') and after 30 min of reperfusion (pP30') with and without 600 mg LA 15 min prior to ischemia. Positive control was prepared from liver-tissue samples by treating with DNase I in accordance with manufactures guidelines (E). After LAP TUNEL-positive hepatocytes were rare (data not shown). We observed TUNEL-positive hepatocytes in the untreated group at pP0' and at pP30' (B, D) while TUNEL staining was decreased in the LA pretreated group at the same time point (A, C); F: TUNEL-positive hepatocytes after laparotomy (LAP), after 30 min of ischemia (pP0') and after 30 min of reperfusion (pP30') with and without 600 mg LA 15 min prior to ischemia, values as means ± SD, n = 5 each group, bP < 0.01 vs vehicle (NaCl) group.