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Urea synthesis by differentiated cES cells. The level of urea nitrogen in the culture medium of cES cells cultured with MFLCs was greater than that in the medium of cES cells subjected to GF-induction, though amounts of urea synthesized by cES cells co-cultured with MFLCs scarcely increased from d 14 to 28. Spontaneously differentiated cES cells showed nearly no ability to synthesize urea.
