Table 2.
Relative luminescence units (RLU) of cytosolic adenylate kinase released from the hepatoma cell line HepG2 for HCC patients and the colorectal carcinoma cell line CaCo for patients with colorectal carcinoma metastatic to the liver
| Tumor entity | CP | Etiology of | AK release before | AK release 4 wk | AK release 8 wk |
| cirrhosis | RFA | after RFA | after RFA | ||
| HCC 1 | A | alcohol | 4.8 | 398.46 | 535.87 |
| HCC 2 | A | Hep C | 3.26 | 421.66 | 645.9 |
| HCC 3 | B | Hep C | 4.88 | 578.63 | 845.99 |
| HCC 4 | A | alcohol | 3.95 | 865.66 | 1025.21 |
| HCC 5 | B | alcohol | 5 | 465.7 | 732.13 |
| HCC 6 | A | alcohol | 4.66 | 751.33 | 924.66 |
| Mean | 4.42 | 580.24b | 784.96b | ||
| (SD) | 0.68 | 190.81 | 181.73 | ||
| Tumor entity | Primary | AK release before | AK release 4 wk | AK release 8 wk | |
| RFA | after RFA | after RFA | |||
| CRC metastasis 1 | Sigma | 2.13 | 568.32 | 816.55 | |
| CRC metastasis 2 | Colon | 3 | 947.47 | 1298.25 | |
| CRC metastasis 3 | Colon | 2.79 | 627.55 | 945.33 | |
| CRC metastasis 4 | Sigma | 2.56 | 699.45 | 845.54 | |
| CRC metastasis 5 | Rectum | 2.98 | 598.65 | 932.17 | |
| CRC metastasis 6 | Colon | 2.27 | 621.46 | 889.66 | |
| Mean | 2.62 | 677.15b | 954.58b | ||
| (SD) | 0.37 | 139.38 | 175.42 | ||
Cells were co-incubated with PBMC isolated from each patient taken before, 4 and 8 wk after RFA. CP: Child Pugh-Score of patients with liver cirrhosis; LC: liver cirrhosis; HCC: hepatocellular carcinoma; CRC metastasis: patients with colorectal cancer metastastatic to the liver; Primary: location of the primary tumor; m.d.: missing data;
b
P < 0.0001 vs bioluminescence before RFA.