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NF-κB-driven SEAP activity in the supernatants of HT-29 clone 34 cells pre-incubated with different bifidobacteria and subsequent stimulation with TNF-α (10 μg/L) for 16 h in the presence of 50 mL/L HM. Cells treated with medium only served as negative control (neg) and positive controls (pos) were cells challenged with TNF-α + HM without bacterial pre-treatment. Results are given as RLU and are means ± SD of triplicate measurements of a representative of two independent experiments.
