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Electrophoretic analysis of AFU in crude and purified human liver. Lane 1: 50% to 60% (NH4)2SO4 precipitation; lane 2: supernatant after high speed centrifugation; lane3: protein standards(Fermentas , prestained); lane 4: 35% to 50% (NH4)2SO4 precipitation; lane 5: ultrafiltration fraction after 35% to 50% (NH4)2SO4 precipitation; lane6: CM-cellulose fraction; lane 7: Homogenate and incubate at 37°C.
