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Western blot analysis showing that purified AFU could recognize a single subunit of 5 Ku (transverse arrow of the enzyme at 30s, 2 min, 5 min, respectively. Lane 1: purified AFU (5 μg) detected with a 103 dilution of antiserum; lane 2: purified AFU (10 μg) detected with a 5×102 dilution of antiserum; lane 3: purified AFU (5 μg) detected with a 5×102 dilution of antiserum.
