Target 1: 5´-ggaagagaacaggactgaggc-3´ (Target site: 90-110) |
siRNA- expressing vector No.1 |
Oligo 1 F: 5´-tcgaggaagagaacaggactgaggcttcaagagagcctcagtcctgttctcttccttttt-3´ |
Oligo 1 R: 5´-ctagaaaaaggaagagaacaggactgaggctctcttgaagcctcagtcctgttctcttcc-3´ |
Target 2 :5´- gaacaggtagtgaatgaactc-3´(Target site: 370-390) |
siRNA- expressing vector No.2 |
Oligo 2 F: 5´-tcgagaacaggtagtgaatgaactcttcaagagagagttcattcactacctgttcttttt-3´ |
Oligo 2 R: 5´-ctagaaaaagaacaggtagtgaatgaactctctcttgaagagttcattcactacctgttc-3´ |
Target 3: 5´- gaacgcttcaaccgctggttc-3´(Target site: 622-642) |
siRNA-expressing vector No.3 |
Oligo3 F: 5´-tcgagaacgcttcaaccgctggttcttcaagagagaaccagcggttgaagcgttcttttt-3´ |
Oligo 3 R: 5´-ctagaaaaagaacgcttcaaccgctggttctctcttgaagaaccagcggttgaagcgttc-3´ |
Control: 5´-gaggaccgttactagatcata-3´ |
Control siRNA- expressing vector |
Oligo F: 5´-tcgagaggaccgttactagatcatattcaagagatatgatctagtaacggtcctcttttt-3´ |
Oligo R: 5´-ctagaaaaagaggaccgttactagatcatatctcttgaatatgatctagtaacggtcctc-3´ |