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. 2006 Aug 14;12(30):4850–4858. doi: 10.3748/wjg.v12.i30.4850

Figure 2.

Figure 2

DA-9601 inhibits the expression and secretion of CCL20 in AGS cells. (A) AGS cells (1 x 105) were treated with various concentrations of DA-9601 (0-100 μg/mL) for 16 h. Quantitative analysis of cell viability was determined by the MTT assay (mean ± SD., n = 3). (B) Cells (5 x 105) were pretreated with various concentrations of DA-9601 (0-100 μg/mL) for 1 h, and then the cells were further incubated for 8 h with TNF-α (5 ng/mL). Levels of IL-8 and CCL20 mRNAs were determined by RT-PCR. (C) Cells (5 x 105) were pretreated with various concentrations of DA-9601 (0-100 μg/mL) for 1 h, and then the cells were further incubated for 16 h with TNF-α (5 ng/mL). IL-8 and CCL20 protein levels were determined by ELISA. These data are representative of three independent experiments.