Figure 6.
SB203580 blocks NF-κB-dependent transcriptional activity in AGS cells. AGS cells (5 x 105) were transfected with NF-κB luciferase reporter vector (0.8 μg/well). After 24 h of incubation, cells were pre-treated for 1 h with PD098059 (20 μmol/L), SB203580 (10 μmol/L), SP600125 (2 μmol/L), and PDTC (10 μmol/L); the cells were stimulated for additional 16 h with medium alone or medium containing TNF-α (5 ng/mL). At the end of incubation, cells were lysed, and the relative luciferase activity was measured using Luciferase Assay System. Note, aP < 0.05, significantly different from control (n = 4).