Swollen mitochondria, ruptured crista, rough endoplasmic reticulum degranulation (× 15 000) in the group undergoing warm ischemia injury for 30 min (A); phanero-swollen mitochondria and nuclear chromosome margination, and karyopyknosis after reperfusion (× 10 000) in the group undergoing warm ischemia injury for 45 min (B and C); significant swollen mitochondria, crista extinction, nuclear membrane rupture, karyolysis and karyorrhexis after reperfusion (× 5000) in the group undergoing warm ischemia injury for 60 min (D).