Table 1.
Gene | Primer Sequence | T (°C) | |
HGF | Sense | 5’-TTATGGGGAATGAGAAATGC | 60 |
Antisense | 5’-TCGAACAAAAATACCAGGAC | ||
c-Met | Sense | 5’-CAGACGCCTTGTATGAAGT | 60 |
Antisense | 5’-CATAAGTAGCGTTCACATGG | ||
TGF-β1 | Sense | 5’-ATGACATGAACCGACCCTTC | 60 |
Antisense | 5’-TGTGTTGGTTGTAGAGGGCA | ||
VEGF | Sense | 5’-AATTGAGACCCTGGTGGACA | 56 |
Antisense | 5’-TAGTGACGTTGCTCTCCGAC | ||
GAPDH | Sense | 5’-TGAACGGGAAGCTCACTGG | 60 |
Antisense | 5’-TCCACCACCCTGTTGCTGTA | ||
β-actin | Sense | 5’-CCTGTATGCCTCTGGTCGTA | 60 |
Antisense | 5’-CCATCTCTTGCTCGAAGTCT |
T : annealing temperature .