Hematoxylin-eosin staining of the surgical specimens (A, C, and E). The images scanned and depicted only blue signals using a computer program. The blue signals were calculated as the area of fibrosis (B, D, and F). The results of such fibrosis were 3.2% for B, 12.2% for D, and 27.7% for F. The bar shows 100 μm.