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. 2006 Nov 28;12(44):7118–7125. doi: 10.3748/wjg.v12.i44.7118

Figure 2.

Figure 2

Expression of Flt3-L in transiently transfected HuH-7 cells: (A) Detection of mouse Flt3-L on a 150 g/L SDS-PAGE using an anti-His-Tag antibody. Different glycosylation forms of Flt3-L in cell were detected in cell lysates and supernatants. Lane 1 and 3: mock-transfected cells; lane 2 and 4: Flt3-L-transfected cells; lane 1 and 2: supernatants after transient transfection; lane 3 and 4: cell lysates. (B) To confirm these results, supernatants and cell lysates were incubated by an Flt3-L-specific ELISA. Again, secretion levels into the supernatant were more than 20-fold higher compared to levels seen in the cell compartment.