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ELISPOT assay: Assessment at the individual single cell level of the number of mIFN-γ-secreting cells as means of CD8 T cell function. Spleen cells were stimulated with recombinant HCV-NS5 protein at 0.1 and 1 µg/mL, and the number of spot forming units/106 PBMC was counted after 24 h incubation period (n = 10 mice/group). Note that lymphocytes were not restimulated in NS5-expressing cells.
