Table 1.
Patient | Addition of T3 |
CA |
CDCA |
Ratio |
ng/mL | Medium/24 h | CA/CDCA | ||
A | Control | 920 | 595 | 1.5 |
0.1 nmol/L | 400 | 310 | 1.3 | |
1 nmol/L | 375 | 375 | 1.0 | |
10 nmol/L | 340 | 390 | 0.9 | |
100 nmol/L | 250 | 285 | 0.9 | |
1 μmol/L | 245 | 295 | 0.8 | |
B | Control | 280 | 60 | 4.7 |
0.1 nmol/L | 135 | 45 | 3.0 | |
1 nmol/L | 95 | 70 | 1.4 | |
10 nmol/L | 55 | 60 | 0.9 | |
100 nmol/L | 45 | 65 | 0.7 | |
1 μmol/L | 45 | 70 | 0.6 | |
C | Control | 805 | 502 | 1.6 |
0.1 nmol/L | 430 | 345 | 1.2 | |
1 nmol/L | 477 | 392 | 1.2 | |
10 nmol/L | 395 | 425 | 0.9 | |
100 nmol/L | 372 | 405 | 0.9 | |
1 μmol/L | 382 | 420 | 0.9 |
The cells were cultured in a serum-free medium and treated with different concentrations of T3 for 5 d. Bile acids were measured in the cell-free medium. CA: Cholic acid; CDCA: Chenodeoxycholic acid.