Table 1.

QRT-PCR parameters

Gene	Primer	Sequence	Length of primer (bp)	Length of product (bp)	Location of product
HSE1
	Forward prime	5’-TGGCAGGACAAGCGTTTAGA-3’	20	66	43–108
	Reverse prime	5’-CAGCACTCCTTTCAACCAATACTC-3	24
	TaqMan probe	5’>FAM AGTTTCTTCCACTCTTTG Quencher < 3’	18		64–81
SOX9
	Forward prime	5’-AGCGACGTCATCTCCAACATC-3’	21	49	1235–1
	Reverse prime	5’-GTTGGGCGGCAGGTACTG-3’	18		252
	TaqMan	5’>FAM CCTTCGATGTCAACGAGT Quencher < 3’	18
CaM					3098–3
	Forward prime	5’-GTTGAGCGAGGCAAATGGAT-3’	20	62	161
	Reverse prime	5’-TCCTTGGCAACAGTGCATCA-3’	20
	TaqMan	5’>FAM TCGATATTTCAGATGGGC Quencher <3 ’	18
β-Actin					1058–1
n	Forward prime	5’-CTGGCACCCAGCACAATG-3’	18	93	075
	Reverse prime	5’-GGACAGCGAGGCCAGGAT-3’	18
	TaqMan	5’>FAM ATCATTGCTCCTCCTGAG Quencher < 3’	18