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Southern blot hybridization analysis of nested PCR amplicons derived from ss-cDNA 1 to 26 as specified in Table 1. Lane 27 represents a negative PCR control (no ss-cDNA template added to the PCR master mix). Exposure to X-ray films was for 1 d using an intensifier screen at -70 °C. A: hGRP-PCR amplicons; B: hGRP receptor PCR amplicons.
