Figure 2. Norrin blocking antibody reduces the branch point number (1/mm²) and the network length (mm/mm²) of endothelial cells during in vitro angiogenesis.

Panel (A). Time lapse images of antiogenesis under control contitions from 0 to 120 hours is shown. Green fluorescent protein-stained primary endothelial cells form vessel-like structures that can be maintained up to 120 hours until medium resources are depleted. Panel (B). In vitro angiogenesis at 40 hours in the presence of anti-Norrin antibody, showing markedly reduced vessel formation compared to control. Panel (C). Quantitative representation of the effect of anti-Norrin antibody on branch point number (1/mm²) over 160 hours. Anti-Norrin antibody reduces the branch point number during in vitro angiogenesis. Panel (D). Quantitative representation of the effect of anti-Norrin antibody on network length (mm/mm²) over 160 hours. Anti-Norrin antibody reduces the network length during in vitro angiogenesis.