Table 2. List of strains used in this study with their relevant characteristics.
| Strain No | Host | Plasmid(s) | Relevant characteristics | Reference |
|---|---|---|---|---|
| 97 | E. coli BL21 (DE3) | pLysS | Host strain for overexpression from the T7 promoter | 40 |
| 3325 | E. coli BL21 (DE3) pLysS | pAR2 | Host 97, cytoplasmic overexpression of His6-tagged TNT.R3 | This study |
| 3725 | E. coli BL21 (DE3) pLysS | pAR1 | Host 97, cytoplasmic overexpression of His6-tagged RbsB | This study |
| 4076 | Escherichia coli BW25113 ΔrbsB | rbsB | 42 | |
| 4175 | E. coli BW25113 ΔrbsB | pAR3 pSYK1 | expression of His6-tagged RbsB with signal sequence from PAA expression of TrzI, gfpmut2 fusion to ompC promoter | This study |
| 4176 | E. coli BW25113 ΔrbsB | pAR4, pSYK1 | as 4175, but expressing the TNT.R3 mutant protein | This study |
| 4497 | E. coli BW25113 ΔrbsB | pSTV28, pSYK1 | as 4175, but with empty vector. | This study |
| 4500 | E. coli BW25113 ΔompF | ompF | 42 | |
| 4501 | E. coli BW25113 Δtrg | trg | 42 | |
| 4502 | E. coli BW25113 ΔfliC | fliC | 42 | |
| 4503 | E. coli BW25113 ΔompC | ompC | 42 | |
| 4504 | E. coli BW25113 ΔenvZ | envZ | 42 | |
| 4505 | E. coli BW25113 ΔrbsK | rbsK | 42 | |
| 4515 | E. coli BW25113 ΔompF | pAR3, pSYK1 | as 4175 in host 4500 | This study |
| 4516 | E. coli BW25113 Δtrg | pAR3, pSYK1 | as 4175 in host 4501 | This study |
| 4517 | E. coli BW25113 ΔfliC | pAR3, pSYK1 | as 4175 in host 4502 | This study |
| 4518 | E. coli BW25113 ΔompC | pAR3, pSYK1 | as 4175 in host 4503 | This study |
| 4519 | E. coli BW25113 ΔenvZ | pAR3, pSYK1 | as 4175 in host 4504 | This study |
| 4520 | E. coli BW25113 ΔrbsK | pAR3, pSYK1 | as 4175 in host 4505 | This study |