Table 3. Occurrence of RbsB-His6 and TNT.R3-His6 in E. coli BW25113 ΔrbsB (pSYK1) protein fractions.
| Periplasmic protein fraction1 | Whole soluble protein fraction1 | |||||
|---|---|---|---|---|---|---|
| Identified protein(s) | Strain 4175 RbsB-His6 | Strain 4176 TNT.R3-His6 | Strain 4497 Ctrl | Strain 4175 RbsB-His6 | Strain 4176 TNT.R3-His6 | Strain 4497 Ctrl |
| RbsB-His6 | 18 (8)2 | 5 (5) | 4 (4) | 14 (5) | 1 (1) | 1 (1) |
| TNT.R3-His6 | ND3 | ND | ND | ND | 2 (2) | ND |
| RbsB-His6 + TNT.R3-His64 | 18 (5) | 6 (6) | ND | 10 (5) | 5 (3) | ND |
| Total RbsB-His6 + TNT.R3-His65 | 36 (13) | 11 (11) | 4 (4) | 24 (10) | 8 (6) | 1 (1) |
| Total proteins | 116 (387)6 | 86 (277)6 | 104 (349)6 | 259 (1273)6 | 234 (971)6 | 254 (1061)6 |
1) 28–36 kD fragments on SDS-PAGE gel from extracts as shown in Fig. 2C, originating from E. coli BW25113 ΔrbsB (pSYK1) expressing rbsB-his6 (from plasmid pAR3, strain 4175), E. coli BW25113 ΔrbsB (pSYK1) expressing tnt.R3-his6 (from plasmid pAR4, strain 4176), or E. coli BW25113 ΔrbsB (pSYK1) carrying empty pSTV (strain 4497, Ctrl).
2) Normalized number of peptide mass fragments identifying the protein(s) as total number of specific peptide fragments by the averaged total peptide count in the sample; between brackets, number of unique peptide mass fragments identifying the protein(s).
3) ND, not detected.
4) Peptides covering regions common to both RbsB-His6 and TNT.R3-His6.
5) All peptides identifying both RbsB-His6 and TNT.R3-His6.
6) Total number of identified proteins within the 27–32 kDa purified gel region; within brackets: total number of identified peptide mass fragments.