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Detection of 5’UTR-215 G/A heterozygote in SPINK1 gene by BglI restriction endonuclease cleavage. 1: untreated prF-1R PCR fragment; 2: normal (-215 G/G) prF-1R PCR fragment treated with BglI endonuclease; 3: heterozygote (-215 G/A) prF-1R PCR fragment treated with BglI endonuclease [M: marker (Boehringer-Mannheim marker VIII); bp: base pairs].
