Table 3.
Reactivity of mutant HBs antigens secreted by transfected cells as determined by three commercial ELISA kits
| Expressed |
Bioelisa |
ETI-MAK-4 |
Hepanostika |
||||
| HBsAg | A450 | Index | A450 | Index | A450 | Index | Conc. |
| Gly D | > 3 | > 5.5 | 2.9 | 5.1 | > 3 | > 11 | > 200 |
| Gly Y | 2.97 | 5.5 | 2.97 | 5.2 | > 3 | > 11 | > 200 |
| 91-4696 | > 3 | > 5.5 | 2.87 | 5 | > 3 | > 11 | > 200 |
| SA6 | 2.96 | 5.5 | 2.74 | 4.8 | > 3 | > 11 | > 200 |
| SA7 | > 3 | > 5.5 | 2.87 | 5 | > 3 | > 11 | > 200 |
| 1056 | 1.5 | 2.8 | 1.8 | 3.2 | 2.32 | 8.6 | 162 |
| PA17D | 1 | 1.8 | 2.7 | 4.7 | > 3 | > 11 | > 200 |
| M5 | 1.59 | 2.9 | 0.74 | 1.3 | 1.39 | 5.1 | 92 |
| BA2.4 | 1.66 | 3.1 | 2.08 | 3.6 | 2.33 | 8.6 | 162 |
| SA4 | 1.67 | 3.1 | 1.34 | 2.3 | 2.12 | 7.8 | 148 |
| BA3.2 | 0.54 | 1 | 0.65 | 1.1 | 1 | 3.7 | 62 |
| pRK5 | 0.17 | 0.31 | 0.08 | 0.15 | 0.1 | 0.37 | 0 |
| Medium | 0.18 | 0.33 | 0.11 | 0.19 | 0.1 | 0.37 | 0 |
Results are expressed as absorbance (A) measured at 450 nm, index (ratio of absorbance of mutant sample to absorbance obtained for 1.25 ng/mL of standard adw HBsAg) and concentration (Conc.) presented as ng/mL. Concentration was measured only by the Hepanostika kit using the standard adw HBsAg to construct the standard curve.