Figure 6.

Dose-dependent effect of extracellular Ca²⁺ on MMP release. 8-bromo-cGMP (2 mmol/L) pre-treated and not pretreated 3T3 cells were plated in media with serial doses of Ca²⁺ (0, 0.09 ,0.4, 0.8, 1.2 mmol/L) and incubated for 16-18 h, 200 μL conditioned media was used as sample. The 72 kDa gelatinolytic bands corresponded to the MMP-2, the 92 kDa band to MMP-9. A: 3T3 cell mean ratio of 72 kDa in groups 1-5 was 0.198 ± 0.094, 0.287 ± 0.114, 0.486 ± 0.079, 1.247 ± 0.089 and 2.487 ± 0.179, respectively (n = 4-6); B: 8-bromo-cGMP (2 mmol/L) pre-treated 3T3 cell mean ratio of 72 kDa in groups 1-5 was 0.104 ± 0.084, 0.126 ± 0.086, 0.257 ± 0.102, 0.490 ± 0.173 and 0.784 ± 0.187, respectively (n = 4-6).