Skip to main content
. Author manuscript; available in PMC: 2014 Jul 9.
Published in final edited form as: Methods Enzymol. 2013;529:209–226. doi: 10.1016/B978-0-12-418687-3.00017-3

Table 17.2.

Cell lines and growth conditions

Cell line Ideal growth condition Optimal media Cell source
HEK293S Gnti- Suspension Freestyle293 ‘completed’ ATCC CRL3022
HEK293S Gnti- Adherent DMEM/F12, 5% FBS ATCC CRL3022
HEK293T/17 Adherent DMEM/F12, 5% FBS ATCC CRL11268
CHO-S Suspension Hybridoma, 1% FBS INVITROGEN R800-07
CHO-S Adherent DMEM/F12, 5% FBS INVITROGEN R800-07
CHO dhfr– Adherent Alpha MEM, 5% FBS, HT ATCC CRL-9096
LEC1 Adherent Alpha MEM, 5% FBS ATCC CRL-1735
COS7 Adherent DMEM/F12, 5% FBS ATCC CRL1651