| Component | Final Concentration | Stock | Amount |
|---|---|---|---|
| Methotrexate | 30 mM | 0.136 g | |
| Alpha MEM | 9.5 ml | ||
| NaOH | 50 mM | 1 M | 0.5 ml |
Mix well until MTX is dissolved and pass through a 0.2 μm filter to sterilize. Store in sterile aliquots at −20 −C
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.