Table 2.
Primers used for preparation of mutated constructs of CYP3A4-P92-luc reporter gene The underlined letters indicated the restriction sites and highlight letters indicated mutant sites.
| Primer | Sequence(5’-3’) |
|---|---|
| CYP3A4(−92/−88Xhols) | ATACTACTCGAGCGTCCCAGAGCACAGGTGGCCCTGCTACTGGCTGCAGCTCCAGCCCTGCCTCCTTCTCTA |
| CYP3A4(−87/−83Xhols) | ATACTACTCGAGGCAGGGTCTCCACAGGTGGCCCTGCTACTGGCTGCAGCTCCAGCCCTGCCTCCTTCTCTA |
| CYP3A4(−82/−78Xhols) | ATACTACTCGAGGCAGGCAGAGGTGTCGTGGCCCTGCTACTGGCTGCAGCTCCAGCCCTGCCTCCTTCTCTA |
| CYP3A4(−77/−73Xhols) | ATACTACTCGAGGCAGGCAGAGCACAGCACCGCCTGCTACTGGCTGCAGCTCCAGCCCTGCCTCCTTCTCTA |
| CYP3A4(−72/−68Xhols) | ATACTACTCGAGGCAGGCAGAGCACAGGTGGCGGACGTACTGGCTGCAGCTCCAGCCCTGCCTCCTTCTCTA |
| CYP3A4(−67/−63Xhols) | ATACTACTCGAGGCAGGCAGAGCACAGGTGGCCCTGCATGACGCTGCAGCTCCAGCCCTGCCTCCTTCTCTA |
| CYP3A4(−62/−58Xhols) | ATACTACTCGAGGCAGGCAGAGCACAGGTGGCCCTGCTACTGCGACGAGCTCCAGCCCTGCCTCCTTCTCTA |
| CYP3A4(+4HindIIIa) | ATACTAAAGCTTCAGTGATTCAGTGAGGCTGTTGGATTGTTTATATGCTAGAGAAGGAGGCAGGGCTGGAGCT |
| CYP3A4(−57/−53Xhols) | ATACTACTCGAGGCAGGCAGAGCACAGGTGGCCCTGCTACTGGCTGCTCGAGCAGCCCTGCCTCCTTCTCTA |
| CYP3A4(−57/−53HidIIIa) | ATACTAAAGCTTCAGTGATTCAGTGAGGCTGTTGGATTGTTTATATGCTAGAGAAGGAGGCAGGGCTGCTCGA |
| CYP3A4(−52/−48Xhols) | ATACTACTCGAGGCAGGCAGAGCACAGGTGGCCCTGCTACTGGCTGCAGCTCGTCGGCTGCCTCCTTCTCTA |
| CYP3A4(−52/−48HidIIIa) | ATACTAAAGCTTCAGTGATTCAGTGAGGCTGTTGGATTGTTTATATGCTAGAGAAGGAGGCAGCCGACGAGCT |
| CYP3A4(−47/−43Xhols) | ATACTACTCGAGGCAGGCAGAGCACAGGTGGCCCTGCTACTGGCTGCAGCTCCAGCCGACGGTCCTTCTCTA |
| CYP3A4(−47/−43HidIIIa) | ATACTAAAGCTTCAGTGATTCAGTGAGGCTGTTGGATTGTTTATATGCTAGAGAAGGACCGTCGGCTGGAGCT |
| CYP3A4(−42/−38Xhols) | ATACTACTCGAGGCAGGCAGAGCACAGGTGGCCCTGCTACTGGCTGCAGCTCCAGCCCTGCCAGGAACTCTA |
| CYP3A4(−42/−38HidIIIa) | ATACTAAAGCTTCAGTGATTCAGTGAGGCTGTTGGATTGTTTATATGCTAGAGTTCCTGGCAGGGCTGGAGCT |
| CYP3A4(−37/−33Xhols) | ATACTACTCGAGGCAGGCAGAGCACAGGTGGCCCTGCTACTGGCTGCAGCTCCAGCCCTGCCTCCTTGAGAT |
| CYP3A4(−37/−33HidIIIa) | ATACTAAAGCTTCAGTGATTCAGTGAGGCTGTTGGATTGTTTATATGCATCTCAAGGAGGCAGGGCTGGAGCT |
| CYP3A4(−92Xhols) | ATACTACTCGAGGCAGGCAGAGCACAGGTGGCCCTGCTACTGGCTGCAGCTCCAGCCCTGCCTCCTTCTCTA |
| CYP3A4(−32/−28HidIIIa) | ATACTAAAGCTTCAGTGATTCAGTGAGGCTGTTGGATTGTTTAATACGATCTCAAGGAGGCAGGGCTGGAGCT |
| CYP3A4(−27/−23HidIIIa) | ATACTAAAGCTTCAGTGATTCAGTGAGGCTGTTGGATTCAAATTATGCATCTCAAGGAGGCAGGGCTGGAGCT |
| CYP3A4(−22/−18HidIIIa) | ATACTAAAGCTTCAGTGATTCAGTGAGGCTGTTCCTAAGTTTATATGCATCTCAAGGAGGCAGGGCTGGAGCT |
| CYP3A4(−17/−13HidIIIa) | ATACTAAAGCTTCAGTGATTCAGTGAGGGACAAGGATTGTTTATATGCATCTCAAGGAGGCAGGGCTGGAGCT |