Figure 1.

Effects of metergoline on neuronal Nav1.2 channel currents and the current-voltage relationship. (A–C) Oocytes were injected with wild-type Nav1.2 α- and β1-subunit cRNA and maintained for three to four days before Na⁺ currents were recorded in ND96 using the two-electrode voltage clamp technique. The traces are representative of six separate oocytes from three different frogs in the absence (Con) or presence of 10 μmol/L metergoline or washout. Metergoline showed substantial inhibition on the peak currents of Nav1.2. (D) The current-voltage relationship was obtained using voltage steps between −50 mV and +50 mV taken in 5-mV increments. Voltage steps were applied in the absence (•) or presence (○) of 10 μmol/L metergoline or after washout of metergoline (▾). The peaks of the evoked currents, normalized to the peak current evoked by the voltage step to −10 mV in the absence of metergoline, were used in the I–V plot. The data represent the mean±SEM (n=7–9/group).