Table 2. mRNA level of the genes during differentiation. Besides GAPDH mRNA levels, all data were normalized to GAPDH first and then normalized to d 0 mRNA levels and presented as mean fold change±SD (n=2/group). GAPDH mRNA levels were normalized to its d 0 mRNA levels and presented as mean fold change±SD (n=2/group). The cycle threshold value (CT) value of RT-PCR was also provided as mean Ct value±SD.
| Gene | Mean fold change versus d 0±SD (means Ct value±SD) |
||
|---|---|---|---|
| d 0 | d 2 | d 6 | |
| GAPDH | 1.00±0.02 (17.91±0.27) | 0.47±0.01 (18.90±0.27) | 0.35±0.05 (19.52±0.32) |
| KCNJ6 | 1.00±0.14 (33.76±0.48) | 240.52±29.50 (26.83±0.30) | 571.81±98.00 (26.21±0.24) |
| RET | 1.00±0.40 (34.02±0.36) | 1.12±0.22 (34.79±0.31) | 7.04±2.54 (32.81±0.32) |
| AADC | 1.00±0.17 (34.70±0.53) | 0.59±0.42 (36.74±1.11) | 43.33±8.38 (30.88±0.48) |
| Nestin | 1.00±0.24 (26.25±0.64) | 0.10±0.01 (30.53±0.32) | 3.03±0.62 (26.26±0.61) |
| GFRA1 | 1.00±0.10 (26.05±0.16) | 1.98±0.43 (26.07±0.31) | 32.27±2.89 (22.65±0.36) |
| SYN1 | 1.00±0.33 (34.80±0.75) | 540.60±90.69 (26.66±0.33) | 4083.39±384.86 (24.35±0.44) |
| EN1 | 1.00±0.12 (24.90±0.13) | 0.38±0.08 (26.34±0.35) | 0.77±0.22 (25.29±0.26) |
| SYP | 1.00±0.12 (27.37±0.22) | 13.87±0.47 (24.55±0.14) | 25.95±2.80 (24.28±0.16) |
| DRD2 | 1.00±0.13 (30.28±0.16) | 235.89±29.03 (23.48±0.34) | 1407.76±60.46 (21.36±0.23) |
| PITX3 | 1.00±0.50 (32.15±0.80) | 0.57±0.26 (33.99±0.67) | 1.18±0.58 (33.45±0.47) |
| VMAT2 | 1.00±0.57 (31.05±0.87) | 1.45±0.14 (31.45±0.09) | 4.81±0.51 (30.19±0.28) |
| TUJ1 | 1.00±0.09 (22.29±0.34) | 15.93±1.62 (19.38±0.26) | 21.90±2.20 (19.39±0.36) |
| NURR1 | 1.00±0.20 (26.36±0.26) | 1.99±0.17 (26.43±0.30) | 0.92±0.08 (28.00±0.15) |
| DAT | 1.00±0.07 (28.63±0.19) | 43.12±15.70 (24.36±0.63) | 3.04±0.29 (28.57±0.36) |
| TH | 1.00±0.73 (33.63±0.91) | 0.48±0.09 (35.49±0.43) | 56.33±8.15 (29.06±0.39) |
| GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; KCNJ6, Potassium inwardly-rectifying channel, subfamily J, member 6; RET, Rearranged during transfection; AADC, Aromatic L-amino acid decarboxylase; GFRA1, GDNF family receptor alpha-1; SYN1, Synapsin I; EN1, Engrailed-1; SYP, Synaptophysin; DRD2, Dopamine D2 receptor; PITX3, Pituitary homeobox 3; VMAT2, Vesicular monoamine transporter 2; TUJ1, Tubulin beta-3; NURR1, Nuclear receptor related 1; DAT, dopamine transporter; TH, Tyrosine hydroxylase. | |||