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. 2014 Jul 3;35(7):899–906. doi: 10.1038/aps.2014.40

Figure 3.

Figure 3

Characteristics of KDM expressions in mesodermal cells. (A) Schematic diagram of analysis strategy. (B) Expression of Brachyury-GFP in d 3.5 EBs. Bar=100 μm. (C) FACS sorting of Brachyury− and Brachyrury+ subpopulations at differentiation d 3.5. (D) RT-PCR analysis of Brachyury expression in unsorted, Brachyury− and Brachyury+ cells. (E) Quantitative expression analysis of KDMs between Brachyury− and Brachyury+ subpopulations. Data are represented as mean±SEM from at least three independent experiments. bP<0.05, cP<0.01 compared with corresponding Brachyury− values.