Table 2. Primer sequences of KDMs for Q-PCR.
Gene | Primer sequence (5′–3′) | AT (°C) | PS (bp) |
---|---|---|---|
Jarid1a/Kdm5a | F-CACAGACCCGCTGAGTTTTAT R-CTTCACAGGCAAATGGAGGTT | 59 | 107 |
Jarid1b/Kdm5b | F-CGCTTGCTGCACCGTTATT R-TACATCAGCCTTAGAAGCCATTTTG | 60 | 72 |
Jarid1c/Kdm5c | F-TGAACGCATTGTTTATCCCTATGA R-TCAAATGGGCGTGTGTTACAC | 59 | 75 |
Jarid1d/Kdm5d | F-TCCATTCGCCCTTTAATATCGA R-CGTCAGATCCTGGCTTCATGT | 60 | 72 |
Jhdm1a/Kdm2a | F-GCTGTTCCTGGTCTGCAGTATCT R-TTCCTACTGCCCACCGAAGA | 60 | 84 |
Jhdm1b/Kdm2b | F-GATGCTGAGCGGTATCATCCG R-GAGACAGCGATCCATGAGCAG | 57 | 147 |
Jhdm1d/Kdm7a | F-GGCAAACCAGTTAAATCTCAGGG R-AGGTTAGAAGGAGTTCGGACAT | 59 | 137 |
Jhdm2a/Kdm3a | F-GCCTTCCAGGTTTGATGATCTG R-TGCCATCTCGCCTGGTGTA | 60 | 65 |
Jmjd1b/Kdm3b | F-TCAATATCCACTGGGTCTGTCG R-TGCCCCTTTGCACACTTCAG | 60 | 142 |
Jmjd2a/Kdm4a | F-CCACCGAGACCTTCTACGAAGT R-TACCACTATGTCCTCAGGATAAAGGTT | 61 | 77 |
Jmjd2b/Kdm4b | F-AGGGACTTCAACAGATATGTGGC R-GATGTCATCATACGTCTGCCG | 60 | 114 |
Jmjd2c/Kdm4c | F-GGCGCCATCCACTTGGT R-CGCTACCTGCTGCTTCACAA | 60 | 65 |
Jmjd3/Kdm6b | F-CCCCCATTTCAGCTGACTAA R-CTGGACCAAGGGGTGTGTT | 59 | 199 |
Lsd1/Kdm1a | F-TGGGACCCAAGTGTCAATTTG R-AGAGCTCACCCCTGCTAGCA | 60 | 64 |
Phf8 | F-CGCCCAACAAATGCTAATCT R-AGAAGTTCCCTCCGAATGCT | 59 | 196 |
Utx/Kdm6a | F-ATCCCAGCTCAGCAGAAGTT R-GGAGGAAAGAAAGCATCACG | 59 | 196 |
F, forward; R, reverse; AT, annealing temperature; PS, product size.