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. 2014 Jul 3;35(7):899–906. doi: 10.1038/aps.2014.40

Table 2. Primer sequences of KDMs for Q-PCR.

Gene Primer sequence (5′–3′) AT (°C) PS (bp)
Jarid1a/Kdm5a F-CACAGACCCGCTGAGTTTTAT R-CTTCACAGGCAAATGGAGGTT 59 107
Jarid1b/Kdm5b F-CGCTTGCTGCACCGTTATT R-TACATCAGCCTTAGAAGCCATTTTG 60 72
Jarid1c/Kdm5c F-TGAACGCATTGTTTATCCCTATGA R-TCAAATGGGCGTGTGTTACAC 59 75
Jarid1d/Kdm5d F-TCCATTCGCCCTTTAATATCGA R-CGTCAGATCCTGGCTTCATGT 60 72
Jhdm1a/Kdm2a F-GCTGTTCCTGGTCTGCAGTATCT R-TTCCTACTGCCCACCGAAGA 60 84
Jhdm1b/Kdm2b F-GATGCTGAGCGGTATCATCCG R-GAGACAGCGATCCATGAGCAG 57 147
Jhdm1d/Kdm7a F-GGCAAACCAGTTAAATCTCAGGG R-AGGTTAGAAGGAGTTCGGACAT 59 137
Jhdm2a/Kdm3a F-GCCTTCCAGGTTTGATGATCTG R-TGCCATCTCGCCTGGTGTA 60 65
Jmjd1b/Kdm3b F-TCAATATCCACTGGGTCTGTCG R-TGCCCCTTTGCACACTTCAG 60 142
Jmjd2a/Kdm4a F-CCACCGAGACCTTCTACGAAGT R-TACCACTATGTCCTCAGGATAAAGGTT 61 77
Jmjd2b/Kdm4b F-AGGGACTTCAACAGATATGTGGC R-GATGTCATCATACGTCTGCCG 60 114
Jmjd2c/Kdm4c F-GGCGCCATCCACTTGGT R-CGCTACCTGCTGCTTCACAA 60 65
Jmjd3/Kdm6b F-CCCCCATTTCAGCTGACTAA R-CTGGACCAAGGGGTGTGTT 59 199
Lsd1/Kdm1a F-TGGGACCCAAGTGTCAATTTG R-AGAGCTCACCCCTGCTAGCA 60 64
Phf8 F-CGCCCAACAAATGCTAATCT R-AGAAGTTCCCTCCGAATGCT 59 196
Utx/Kdm6a F-ATCCCAGCTCAGCAGAAGTT R-GGAGGAAAGAAAGCATCACG 59 196

F, forward; R, reverse; AT, annealing temperature; PS, product size.